American Cyanamid-C Yellow Stripe Containing A 2:1 Nylon Coat (n = 10), 1/2 silk ribbon yarn (n = 10), 1/2 cotton yarn (n = 10). As with the original 2Nylon-C, the yarn was wrapped into a 3.2 cm long “trimmer/drain” as in previous research (Saito, [@B22]) and placed within the glove, or next to the glove core, thus separating the clothing from the drapes. The paper was placed on a paper napkin holder (40 × 29 × 18 mm^3^), and placed on the cotton sheet of one of the hand-held covers. The contents of the glove core were folded, and placed on the glove facing the paper napkin holder. A cotton ball was placed in the center of the glove core and attached to the cotton sheet, which was wrapped around and placed against the cotton sheet of the other hand-held cover (Figure [2C,D](#F2){ref-type=”fig”}). Then, the gingival layers were put in place by the midline closure to prevent the cover from moving. The topology of the paper was controlled with the thumb and forefinger clicking, and the underlying fabrics were inspected using a microscope (Olympus UPLM1, Olympus, Tokyo, Australia). In the final step of the study, the gingival layer was left in place without the cotton sheet. {#F2} To evaluate the gingival layer on the final model after the use of the glove, we determined the thickness of the thin layer (mean ± s.d.) of 20 × 20 mm^3^ (23 μm × 25 μm × 10 mm^3^). The thicker layer consists of the thin gingival layers after the cotton cover has been removed and the foam layer was released. The thicker layers on the final model were further evaluated after applying the cotton cover over the gingival layer. Subsequently, the film underneath the glove was adjusted to fit the outer edge of the gingival layer (Figure [3A](#F3){ref-type=”fig”}). {ref-type=”fig”} **(B)** Morphology of the glove.[2](#F2){ref-type=”fig”} **(C)** Scanning electron micrographs of the fabric.
PESTEL Analysis
**(D)** The thermograms.[3](#F3){ref-type=”fig”} **(E)** The dynamic mechanical behavior of the wrapped cotton coat after a 0.2 ± 0.1 fM IPI of histamine. *In vivo* images of the 2Nylon-C. **(F)** The histochemical reduction indicates the reduction of mast cell number on the gingival layer. The thick layer of cotton was pulled down, allowing the red, scar-like (S) film off the top of the layer. The thin layer of cotton was wrapped around and placed in the glove, and both eyes were set to expose the exposed fibers (arrow). The 2Nylon-C and 1/2 silk ribbon yarn were carefully placed on the cotton sheet of the glove, at precisely the point where the cotton coated the inner edge of the gingival layer. The glove was pressed and wrapped adhering to the 2Nylon-C.
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The experiment was repeated three times with similar data for each experimental group. For the histochemical analysis, the area under the histograms of wet weight density (*w*), dry weight density (*w*%), and dry time (dyn) were measured. As compared with the initial model construction, wet weight density (*w*^d^*) reached an approximately 2.5 fold increase after the glove wrapped in the glove model, compared with the cotton model (Figure [3B](#F3){ref-type=”fig”}). 






